Researchers from CREA have developed an aptamer-based detection tool for agroindustry, allowing for the identification of target strains at a cellular level without the need for nucleic acid extraction. The method, called the ‘systematic evolution of ligands by exponential enrichment’ (SELEX) method, was developed for the detection of Bacillus subtilis, a bacterial species found in soil for plant growth stimulation or protection. The complete genome of the target strain was sequenced to select species-specific aptamers, and a pair of primers were designed for the selection of discriminating aptamer candidates. The technique is transferrable onto mobile devices using a biosensor.
Some conclusions
The successful implementation of modern methods for traceability and monitoring of microbial inoculants in soil is crucial for understanding ecological systems and adopting microbial-based products. This approach promotes environmentally friendly practices, optimizes bioinoculant application, supports regulatory processes, and encourages sustainable cropping systems. Investing in these methods will benefit future generations and preserve the planet’s delicate balance.
