In the fascinating world of soil microbiology, technological advancements and diversity-oriented strategies are transforming our understanding and application of beneficial microorganisms. A prominent example of this progress is the EXCALIBUR Project, which investigates how basic and complementary medium components affect microbial biomass and spore production for formulation into commercial biofertiliser products.
The era of omics and diversity
Nowadays, research in soil, environmental, and human health is shifting towards the use of omics-based and diversity-focused strategies. However, while many studies provide a broad overview of microbial communities, they often lack a deep analysis of the “simple” yet crucial aspects of microbial processes. These often-overlooked elements are key to optimising fermentation and the production of biomass and metabolites, particularly in biofertiliser and biocontrol production.
Optimising fermentation in the EXCALIBUR Project
One of the main focuses of the EXCALIBUR Project is improving the production and formulation stages of biofertiliser products. UGR’s research has concentrated on optimising the fermentation characteristics, including medium composition and fermentation parameters. The aim is to enhance biomass and spore production for subsequent use in product formulation.
Fermentation and formulation strategies
Different fermentation methods have been tested to compare their efficacy. Both submerged liquid fermentation and solid-state fermentation have been employed depending on the characteristics of the microorganisms and the formulation scheme. Each method has its advantages, but the ultimate success relies on maintaining the viability and effectiveness of the products during storage and field application.
Photo 1: Fungal microorganism grown on standard liquid medium and medium supplemented with plant extract.
A crucial part of the research was developing production schemes that significantly increase biomass. By using specific substrates and additives and applying efficient fermentation methods, notable improvements in production were achieved. These optimisations facilitate the next step: product formulation.
Results and Collaborations
The project has enabled the combination of various beneficial microorganisms into a single formulated product, achieving positive effects in combating abiotic and biotic factors. UGR has worked closely with other EXCALIBUR partners, including INOCULUM+ and the Institute of Horticulture in Skiernivice, Poland. Experience has also been exchanged with the Polish company Intermag.
Our findings have been presented at project meetings and events organised by initiatives such as VIRTUOUS, SUSTAINABLE, and the Cooperation Partnership for Digital Higher Education in Integrated Omics for Environmental Sustainability.
Photo 2: The UGR team with participating colleagues at the Digi-Omics Meeting in Granada, June 2024, presenting the EXCALIBUR Project and its links to other projects.
